Sentence examples for modifications of interest from inspiring English sources

Scientists have used the shotgun pro−teomics approach to discover many protein modifications of interest.

The base modifications of interest were the thymine glycol modification of thymine and the formamide modification derived from the breakdown of pyrimidine bases (Bailey et al, 2006; Greene et al, 2007).

Both of these methods involve initially enriching the genome for DNA located around histones of interest by chromatin immunoprecipitation of genomic DNA with antibodies targeted at histone modifications of interest.

However, because they are metabolized by cells before their addition onto proteins, metabolic chemical reporters potentially provide a unique opportunity to read-out on both modifications of interest and cellular metabolism.

Thus, to interrogate truly combinatorial histone modifications, one must either analyze intact histones (i.e. top down) or utilize enzymes that produce longer polypeptides (i.e. middle down) containing the modifications of interest, or the N-terminal tails in the case of histones.

If broad domains of shearing-resistant chromatin exist in the yeast genome, we expected that such regions would be under-represented among the sequence reads in the input controls of ChIP-Seq experiments in which the sheared formaldehyde-crosslinked chromatin has not yet been fractionated by an antibody against a protein or modification of interest.

Histones with the modification of interest were immunoprecipitated by adding 2 μL of antibodies to ~300 500 μL of purified chromatin.

The immunoprecipitation step enriches for chromatin fragments containing a histone modification of interest, whereas the sequencing step is used to quantify the abundance of the underlying DNA.

The data is again shown as the ratio between the percentages of input minichromosomes containing the modification of interest in the treated sample compared to the untreated sample.

Chromatin containing the specific histone modification of interest can be isolated from the rest of the chromatin by being pulled away (immunoprecipitated) using these antibodies.

Protein-DNA interactions are crosslinked in situ followed by immunoprecipitation and sequencing of genome-wide sites associated with a modification of interest.