Sentence examples for modification of interest from inspiring English sources
Similar in part to other biotin switch methodologies, this technique relies on the blocking of native lysine residues and removal of the modification of interest in vitro, after which the newly deacetylated proteins can be captured and identified by mass spectrometry (MS).
If broad domains of shearing-resistant chromatin exist in the yeast genome, we expected that such regions would be under-represented among the sequence reads in the input controls of ChIP-Seq experiments in which the sheared formaldehyde-crosslinked chromatin has not yet been fractionated by an antibody against a protein or modification of interest.
The immunoprecipitation step enriches for chromatin fragments containing a histone modification of interest, whereas the sequencing step is used to quantify the abundance of the underlying DNA.
The data is again shown as the ratio between the percentages of input minichromosomes containing the modification of interest in the treated sample compared to the untreated sample.
Histones with the modification of interest were immunoprecipitated by adding 2 μL of antibodies to ~300 500 μL of purified chromatin.
ChIP-bisulfite-pyrosequencing (ChIP-BS-pyro) allowed us to measure the DNA methylation level associated with a chromatin modification of interest (Fig. 2A).
We also incubated human whole blood with dopamine and did not observe the modifications of interest (Suppl. Fig. 4).
Scientists have used the shotgun pro−teomics approach to discover many protein modifications of interest.
The base modifications of interest were the thymine glycol modification of thymine and the formamide modification derived from the breakdown of pyrimidine bases (Bailey et al, 2006; Greene et al, 2007).
Both of these methods involve initially enriching the genome for DNA located around histones of interest by chromatin immunoprecipitation of genomic DNA with antibodies targeted at histone modifications of interest.
However, because they are metabolized by cells before their addition onto proteins, metabolic chemical reporters potentially provide a unique opportunity to read-out on both modifications of interest and cellular metabolism.
