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Therefore, it has been important to develop a method to visualize histone modifications in living cells and organism.
We thought that the monoclonal antibodies we had generated might be useful for detecting the target modifications in living cells.
FRAP revealed that the binding time of Fabs to the target modifications in living cell nuclei was in a range from <1 to ~30 s, depending on the binding affinity.
Both the template chromatin for transcription and the transcribing enzyme, RNA polymerase, can be heavily modified (Fig. 1) (Bannister and Kouzarides 2011; Eick and Geyer 2013; Kimura 2013; Jeronimo et al. 2013), and our approach to tracking their modifications in living cells has been a breakthrough to fully understand how genes are regulated in vivo.
According to Tidon and Lewontin (2004), teachers usually assure that theories about modifications in living organisms (Darwin and Lamarck) are easy to teach.
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Drug metabolism is a process by which pharmaceutical substances undergo biochemical modification in living organisms.
In other studies, the alkynyl-modified GlcNAc analog (GlcNAlk) has been exploited as a chemical reporter of O-GlcNAc modification in living cells [ 119, 121].
(2018) engineer fluorescent sensors for coexisting histone modifications in live stem cells.
Protein glycosylation is one of the most common post-translational modifications (PTMs) in living cells [ 1, 2].
A subsequent alternative approach that has emerged is the development of azido- and alkyne-functionalized dimedone analogous (Chart 6), termed DAz-1 (15), DYn-1 (17), and DYn-2 (18), which enable the trapping and tagging of protein sulfenic acid modifications directly in living cells.
pBO2pa can form reversible covalent complexes with diols, amino alcohols, and amino acids [18], and may be useful for surface protein modification in live mycobacteria.
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