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For each cell line, we downloaded histone modification data for H3K4me3, H3K27ac and H3K36me3 marks.
A number of hidden Markov model (HMM -based metHMM -basedbeen developed to infer chromethodstate maps from genome-wide haveone modification data for an individual genome.
Let Y = (y 1, …, y T ) be an m × T matrix for histone modification data for m chromatin marks measured at T contiguous locations along the genome.
RNAPII as well as the histone modification data for H3K4me1, H3K4me2, H3K4me3, H3K9ac, H3K9me1, H3K27ac, H3K27me3, H3K36me3, H4k20me1 in all the three cell lines were downloaded from 'Broad Histone' tracks [ 34, 37, 51] in the UCSC browser as mapped reads.
Since the largest available sets of known human MAE and BAE genes were identified in human lymphoblasts (Gimelbrant et al., 2007) (see also Dataset S1 in Dryad, Nag et al., 2013), we used these sets to compare with histone modification data for GM12878 lymphoblastoid cells deposited by the ENCODE project (Dunham et al., 2012).
Once this dataset is released, it will enable the user to search and retrieve post-translational modification data for a protein such as the type of the modification, the modified residue, the coordinate of the modified residue on the protein sequence, the start and stop positions of the protein sequence, and the cellular compartment of the modified protein.
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HMCan was specifically developed to analyze histone modification data obtained for cancer genomes.
We included all available histone modification data sets for four cell lines A549, H1ESC, HSMM, and HSMMT, with the exception of H3K36me3 in A549 because of poor reproducibility of this dataset between replicates.
ChIP-Seq data for histone modification data and Pol II were analysed by using the program SICER version 1.03 [ 63].
Combination of all this information with publicly available expression, DNA methylation and histone modification data can be instrumental for answering questions on epigenetic regulation and inheritance and unveiling long-range patterns of gene regulation and disease development [ 17, 19, 137].
It includes data collection, resolving similarities found in different sources, modification data to a type acceptable for the analysis, the analysis itself and output representation, i.e. the results visualization in a form most suitable for human perception.
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