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In the model, all cells are considered 'active' or 'resting'resting
In the model, all cells divided at each iteration step, but the survival of the daughter cells depended on the local cell density.
First, we notice that we do not need to model all cells in all generations because the CSCs are the only long-lived cells in our tree.
For the cell line panel model, all cells were grown to 70% confluency under normal culture conditions prior to lysis and RNA extraction using the above protocol.
However, in this model, all cells of the mouse express human DPP4.
In our model, all cells within a body are considered to be candidates for acquiring TSG inactivation or oncogene activation.
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Neurons within a patch fire almost synchronously (in our model, all the cells within a patch fire within 4 10 ms of each other, depending upon the size of the patch), but patches discharge at random (see online supplementary movies showing the effects of normal, partial and complete coupling).
The interference from all neighbouring cells at non-negligible distance to the macro cell is also considered in our channel model: all the surrounding cells are configured as the studied macro cell.
In tumor types that do not follow the cancer stem cell model, all cell types may accumulate alterations that have the potential to persist in the population, and therefore all tumor cells are included in the model describing tumors of this latter type.
The focus of our method are studies of signaling pathways which are not adequately modeled in ALL cell lines and require patient-derived tumor cells, such as the p53 network.
Cell survival assay and apoptosis experiment in H2O2 induced PC12 cells injury model all showed that its cytoprotection exhibited a concentration-effect manner.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com