To determine the percentages of each cell pattern, all the cells from four randomly chosen fields of non-necrotic areas of each of the three slices prepared from each tumour were counted.
Particularly interesting are the recent, prognostically relevant findings that noninvasive papillary TCCs may exhibit a normal K20 pattern (predominantly superficial) or an abnormal K20 pattern (all cell layers or negative) and that the normal pattern is predictive of tumor non-recurrence (Southgate et al. 1999).
Under optimal conditions 1G8s stained rat MUC4 inducibly expressed in A375 melanoma cells, revealing a uniform cell surface expression pattern in all cells of the field.
In this strain, RomR-GFP localized in a bipolar, asymmetric pattern in all cells, and all cells harbored the large RomR-GFP cluster at the lagging pole.
Effects of lowered Top2 function on the germline stem cell (GSC) niche were examined by staining with antibodies against Vasa, a germline-specific RNA helicase (Lasko and Ashburner 1988), and Spectrin, a structural protein expressed in a cell-type specific pattern in all cell-type specificrium.
A robust cluster is defined as groups of TSSs which are in close proximity of each other in the genome, have the same direction of transcription, share a similar global expression pattern across all cells and tissues, and have sufficient support in the number of CAGE sequence tags [ 4].
In a, the Ca j patterns in all cells are shown for a period of 100 minutes.
It is noteworthy that specific patterns of bioluminescence signals in the embryo do not represent distribution of the mRNA, but rather real endogenous BMP signals because the same embryo including the Venus mRNA showed almost ubiquitous expression patterns among all cells over time.
Staining patterns in all cells were visually confirmed by assessing the appearance of the cells using the Image Gallery of the Ideas software package.
To further investigate whether, and to what extent, these populations mirror different stages of normal B-cell differentiation, we compared gene expression pattern of primary B-ALL cells sorted for high and low expression of CD34 with those from CD34-positive human haematopoietic stem and progenitor cells and cells of the B-lymphoid lineage.
