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Exact(6)
In contrast, the mobility of mutant ssDNA did not change because the interaction with PEG-b-ODN was negligible.
The mobility of mutant receptors labeled with sQD-mSA1 wasignificantlyly greater than that of labeled wild-type LDL receptor (P = 1.6 × 10−14) [56].
The method is based on the difference in mobility of mutant DNA (in the same length) in PAGE that is due to Zn2+ cyclen binding to thymine bases accompanying a total charge decrease and a local conformation change of target DNA.
The electrophoretic mobility of mutant receptors in SDS-PAGE experiments was indistinguishable from wild type receptors with a predominant band at 60 and 50 kDa for MT1 and MT2, respectively (Figure 1).
In summary, the results of Ferguson plot do not support a hypothesis that different mobility of mutant proteins compared to wild-type CBS is caused by changes in the number of enzyme subunits.
The reason for such change in electrophoretic mobility of mutant RPGRORF15 needs further investigation, but it may result from stable changes in secondary conformation, post-translation modification or both.
Similar(54)
We do, however, observe a change in the mobility shift of mutant Cdc24 proteins on SDS-PAGE, suggesting that we have indeed perturbed its phosphorylation.
Conversion of the Ser114 and Thr115 sites to alanine at the endogenous locus, creating the yox12A mutant strain, results in a dramatic effect on the mobility of the mutant protein in response to HU in vivo (Figure 6B).
In order to gain insights into the pathogenic mechanism of the p.Cys134Trp mutation, the subcellular localization and mobility of the mutant protein were studied and found to be no different from those of the wild type (WT).
They applied sQD-mSA1 to study the mobility of a mutant of low-density lipoprotein (LDL) receptor with a truncated cytosolic tail, originally found from an individual with familial hypercholesterolemia.
In contrast, ERK5 activation did not reduce the electrophoretic mobility of the mutant SA179/208 Neurog1 (Fig. 4 C).
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