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Moreover, when we investigated the mobility of PIPkin Iγ93 D316K by FRAP the mobile fraction was 92% (Table 1), with a computed D of 3.54 μm s−1 (Table 1).

The amount of the mobile fraction was between 0.008 – 31.2 mg/kg, accounting for 0.5 – 36.9% of the total three fractions.

The recovery (mobile fraction) was calculated as described elsewhere.

The mobile fraction was even greater in eco1-1 mutants.

Mobile fraction was determined as Mf = (Ymax − Y0)/(1 − Y0).

The mobile fraction was calculated as the ratio of Fp to the initial fluorescence level (F0).

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mCD8-GFP recovers remarkably quickly (τD=33 s) and its mobile fraction is 40%.

Most of the enzyme was immobile, the mobile fraction being only 36% (Fig. 2C, G and Table 1).

The 15% reduction in the HP1α mobile fraction is consistent with previous data showing a 14 15% increased fraction of very slow HP1α molecules in pericentromeric heterochromatin of prometaphase and metaphase cells (29).

This shows very low levels of recovery in the nucleolus when rRNA synthesis is active (i.e., no actinomycin D treatment), whereas the mobile fraction is clearly increased after blocking rRNA synthesis alone and increased more upon blocking both rRNA synthesis and the proteasome.

The computation of mobile fractions was performed exactly as in Brough et al. [17].

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