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The number of cracks that intersected a grid array of parallel and perpendicular lines that were spaced at 0.1 mm was counted.
The number of elongated cells per field of view (1.376 mm) was counted and averaged.
The number of surviving hippocampal CA1 pyramidal cells per mm was counted as the neuronal density.
The number of tumours >100 mm was counted over the same observation period.
The number of apoptotic (green fluorescence) cells in each field of view (× 40 magnification; 0.55 mm) was counted.
The number of seedlings expressing GUS foci ≥ 2 mm was counted and analyzed using one-way or two-way ANOVA for a fixed effects model (Minitab. 15 software).
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On the morning of Day 0, ovaries were removed, follicles ≥1 mm were counted, and oocytes were collected.
Total number of all visible antral follicles >1 mm were counted and cyclicity status was monitored at monthly interval.
Colonies greater than 2 mm were counted.
Seedlings with GUS foci > 2 mm were counted as positive.
Foci greater then 5 mm were counted manually.
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