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A gallbladder with a diameter of 19 mm was isolated.
For qRT-PCR, frontal cortex (bregma 0 to −2 mm) was isolated using a 'rodent brain matrix' 1 mm coronal slicer (ASI Instruments, Warren, MI) and pooled from three mice of each genotype.
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The sand-sized (ϕ > 0.050 mm) particles were separated through wet sieving and then the clay-sized ones (ϕ < 0.002 mm) were isolated from the silt-sized particles (0.002 mm ≤ ϕ ≤ 0.050 mm) through sedimentation [30].
Several mammary tumors (> 500 mm) were isolated from Hif1a DF, PyMT+ bigenic female mice.
Root tips (5 10 mm) were isolated after 24 hours of exposure to Al and frozen on dry ice.
Only healthy follicles with a diameter of > 5 mm were isolated and snap-frozen in liquid nitrogen [ 2].
From these, anther cones ranging in size from 2-4 mm were isolated, immediately frozen in liquid N2 and pooled for RNA extraction.
Five ug, each, of high quality DNA of DD, F8, and GB at a concentration > 100 ng/ul, (OD 260/280 close to 1.8) in a TE ([EDTA] = 0.1 mM were isolated with the DNeasy Plant Mini kit (Qiagen, CA 91355 Valencia).
Briefly, approximately 2 g of total mitochondrial protein in TAM buffer (30 mM Tris-HCl pH 7.5; 100 mM NH4Cl; 10 mM MgCl2) was isolated by differential centrifugation from approximately 0.5 kg of mycelium.
In order to obtain a partial sequence of the SBV1 genome, total genomic DNA from SBV1 cells grown on MM containing 10 mM β-valine and 20 mM glucose was isolated according to a described procedure (Poelarends et al. 1998).
Differentiated epithelial cells were removed in 1 mM EDTA and 1 mM DTT, where crypt epithelium was isolated in 1 mM EDTA and 5 mM DTT.
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