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QX314 (5 mM) was added to block voltage-gated sodium channels.
To start reaction, NADPH (0.1 mM) was added and incubated at 37 °C for 10 min.
Briefly, a cysteamine solution (400 μl, 213 mM) was added to 40 ml of a 1.42 mM HAuCl4 solution.
Then, freshly prepared NaBH4 (1.65 mL, 5.5 mM) was added.
2.5 µl of probe 1 (1 mM) was added to test wells, and the same volume of DMSO (1 mM) was added to control wells.
Hydrogen peroxide (2 mM) was added to verify the growth against oxidative stress.
Sodium phosphate dodecahydrate (Acros Organics, 8 mM) was added when noted.
FA (5 mM) was added to the medium after 4 and 8 h.
To this solution, freshly prepared sodium borohydride (1.65 mL, 10 mM) was added.
Second, 2 ml HNO3 (0.5 mM) was added into the retrieved solution.
Hydroxylamine (10 mM) was added to quench any unbound EDC/NHS for an additional hour.
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