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Aliquots of this mixture were plated at different time intervals to monitor the increase in phage concentration.
Serial dilutions of the incubation mixture were plated on TH agar, followed by incubation at 37°C overnight and cfu determination.
After peptide exposure, serial dilutions of the incubation mixture were plated on TH agar, followed by incubation at 37°C overnight and cfu determination.
Two layers of agar/media mixture were plated into 60 mm culture dishes.
For immunostaining of cultured cells, WT or KO cells passaged with a dispase-trypsin mixture were plated into tissue culture-treated chamber well slides (BD Biosciences, Franklin Lakes, NJ, USA), grown to 60%to80%0% confluence and then postfixed for 20 minutes at room temperature with 4% paraformaldehyde (PFA -PBS, followed by PFA -PBSlization with 0.5% Triton X-100 followednutes.
Similar(55)
After cultivation overnight at 30°C, the mating mixture was plated onto YPD agar.
400 µl of this mixture was plated per well on top of the solidified matrigel.
Each transformation was performed in triplicate, and the transformation mixture was plated directly on nylon membranes for the lacZ assay.
The cell/virus mixture was plated either 0.5 mL/well in 12-well plates or 0.25 mL/well in 8-well µ-slide (IBIDI) and incubated at 37°C.
A sample (250 µl) of this mixture was plated on an LB-RGK agar medium, and the bacterial titer was calculated as number of colony forming units (CFU).
The cell-agar mixture was plated in duplicate onto dishes containing a solidified.5 ml layer of 0.5% agar-cell culture medium mix.
Related(20)
mixture were spiked
mixture were deposited
compound were plated
mixture were coated
mixture were situated
solution were plated
mixture were placed
mixture were cultivated
mixture were given
mixture were carried
mixture were incubated
mixture were tested
mixture was plated
mixture were investigated
mixture were studied
mixture were observed
mixture were measured
mixture were recorded
mixture were engineered
mixture were captured
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