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The cell mixture was plated on top of a solidified layer of 0.6% agarose in growth medium.
After cultivation overnight at 30°C, the mating mixture was plated onto YPD agar.
400 µl of this mixture was plated per well on top of the solidified matrigel.
Each transformation was performed in triplicate, and the transformation mixture was plated directly on nylon membranes for the lacZ assay.
The cell-agar mixture was plated in duplicate onto dishes containing a solidified.5 ml layer of 0.5% agar-cell culture medium mix.
A sample (250 µl) of this mixture was plated on an LB-RGK agar medium, and the bacterial titer was calculated as number of colony forming units (CFU).
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Transduction to growth on propanediol may require 5 days' incubation at 37 degrees C. 3. In some genetic backgrounds, there is no apparent cotransduction of fuc-3 with argA or rel. 4. It is suggested that as much as 0.1 ml of the undiluted transduction mixture be plated (as well as normal dilutions) as the frequency of transductions may be low.
Aliquots of this mixture were plated at different time intervals to monitor the increase in phage concentration.
Serial dilutions of the incubation mixture were plated on TH agar, followed by incubation at 37°C overnight and cfu determination.
After peptide exposure, serial dilutions of the incubation mixture were plated on TH agar, followed by incubation at 37°C overnight and cfu determination.
Two layers of agar/media mixture were plated into 60 mm culture dishes.
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