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The reaction mixture was periodically sampled.
The reaction mixture was periodically monitored by UV visible spectroscopy to determine the extent of the conversion.
The reaction mixture was periodically analyzed using U-3010 UV-Vis spectropHitachier (Hinachi) in the range of 200 800 nm unless the further increase in absorbance ceased.
The reaction mixture was periodically monitored by UV visible spectroscopy to determine the extent of the conversion, which was found to be complete after 12 h.
An aliquot of this mixture was periodically withdrawn every 15 min for 3 h, and the degree of hemolysis was determined from the concentration of hemoglobin in the supernatant after centrifugation by measuring the absorbance at 524 nm.
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The reaction mixture was withdrawn periodically by using a syringe tube and then centrifuges (REMI, R-8C BL BENCH TOP CENTRIFUGE) to separate the suspended clay particles.
After initiation of the reaction, 100 μL sample of the reaction mixture was withdrawn periodically every 24 h and immediately analyzed by gas chromatography (injection volume = 1 μL) using Perkin Elmer, Model Clarus 500, USA, equipped with a flame ionization detector and RTX-20 (crossband 80%dimethyl-20dimethyl-20%polysiloxane) capillary column (30 m, 0.32 mmID, 1 μm df), Restek, USA.
His work was periodically banned.
The experiment was periodically weeded by hand.
The site was periodically weeded by hand.
To monitor the reaction, samples of the reaction mixture were taken periodically and analyzed by gas chromatography (GC, Perkin Elmer Clarus 500) using a 50 m BP5 capillary column and an free induction decay (FID) detector.
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