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The mixture was constantly stirred for 24 h.
Containers were placed on a coal-fired earthen stove and boiled for 90 min. The mixture was constantly stirred until the content was substantially free of water.
The mixture was constantly stirred for 2 h on ice followed by centrifugation at 100,000 g for 30 min at 4 °C.
Considering that cells may have a difficult time to bind DNA if the cell-DNA mixture was constantly shaken, we attempted to transform static T. sp. strain RQ7 cultures but did not get any transformants.
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The mixtures were constantly stirred at 100 °C until a liquid DES was formed.
The mixtures were constantly stirred under 40 °C for 1 h and then dried by rotary evaporation at 40 °C.
The mixtures were constantly stirred constantly at 40 °C for 15, 30 min, 1, 2, 3, or 4 h and then dried by rotary evaporation at 40 °C.
The mixture was stirred constantly for 2 h by a mechanical stirrer at 100°C.
The mixture was stirred constantly for 2 h at 303 ± 2 K to obtain precipitate of tin (IV) phosphate.
This as-configured mixture was stirred constantly for 30 min, and the color of solution changed from grass green to homogeneous navy blue color.
This reaction mixture was stirred constantly for an hour to ensure that the anions and cation were dispersed homogeneously in the solution, and the color of solution changed from grass-green to navy blue color.
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