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A total of 111 cDNA libraries were excluded from the original set because these libraries lacked clear tissue source information or were from mixed tissue samples.
Previously, 127,094 unigenes were assembled from mixed tissue samples of C. sinensis, with 17.9 % of the unigenes having a length longer than 500 bp [ 17].
Results: We propose a statistical approach to expression deconvolution from mixed tissue samples in which the proportion of each component cell type is unknown.
The mutation occurs in 20 43% of cases (mixed tissue samples), whereas microdissected samples (enriched for tumour cells) of 89% sporadic MSI colon tumours had truncated MBD4 mutations in at least one focus tested (Bader et al, 2000).
To account for the roles of miRNAs in response to GA3 during grapevine berry development, we constructed two small RNA libraries from mixed tissue samples of grapevine berries sprayed with GA3 (treatment) and with water (the control).
The 595 normal sites were divided into adipose (A) (n = 324), mixed tissue samples (Mx) (n = 112), vessel samples (V) (n = 64), fibro-adipose (FA) (n = 60), fibro-glandular (FG) (n = 24), fibrocystic change (FCC) (n = 4), fibrous (F) (n = 6), and fat necrosis (FN) (n = 1) as shown in (Table 1).
Similar(52)
The key component of this method is the estimation, from a mixed tissue sample, of the proportion of mRNA from a single tissue type.
Our method estimates the proportion of each component in a mixed tissue sample; this estimate can be used to provide estimates of gene expression from each component.
For S1, RNA was extracted from a mixed tissue sample (including leaves, stems, stolons and flowers) of above ground parts of a plant grown pot that was placed in darkness for 24 hrs prior to material collection.
Isolation of these putative orthologues in wheat was carried out through direct sequencing of RT-PCR products using cDNA template derived from a mixed tissue sample containing several stages of developing leaves, spikes, carpels and seeds.
A tissue sample from each individual (e.g. a leg; about 2 mm fragment size) was pooled in a single tube containing ethanol and the resultant mixed tissue sample was incubated at 56°C for approximately two hours to evaporate residual ethanol.
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