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The proposed immunosensor is successfully applied to detect MDR cancer cells (MDRCC) in serum and mixed cell samples.
The proposed immunosensor is successfully applied to detect Ep-MCCs in serum and mixed cell samples and interferences due to nontarget cells and molecules present in the real sample matrix are also examined.
To address this issue, studies can be performed either on homogeneous cell populations or on mixed cell samples with deconvolution algorithms applied to correct for differences in cell composition [ 8, 9].
Furthermore, the reported WGA intensity values are just median WGA intensities of a mixed cell samples, rather than of separate mother and daughter populations (reason: overlapping spectral properties of the fluorophores for WGA (bud scar detection) and for Avidin (mother cell detection) made simultaneous use impossible).
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In each of these mixed-cell samples, we determined the fraction of mothers, daughters, and dead cells and generated the mixed-population proteomes and transcriptomes.
Quantification of these populations gives the fractional compositions of each mixed-cell sample (Mix 1 3 in Figure 2 figure supplement 3 ) collected per time point.
The cell counts present in each mixed-cell sample harvested from each time point of the experiment.
(B ) The measured compositions of mother, daughters, and dead cells present in each mixed-cell sample harvested from each time point of the experiment.
The fractional composition of each mixed-cell sample was acquired by using flow cytometry on dye stained cells, using PI and AvF to assess the amount of live mother cells, dead cells, and daughter cells (see: Materials and methods, Flow cytometry analysis of sample composition, and see Figure 2 source data 1 Table S2.f for each time point's matrix).
We were mainly interested in TSSs recognized by the housekeeping sigma factor σA. Therefore the RNA samples for this data set originated from mixed C. glutamicum cell samples grown in minimal and in complex media.
To fully characterize the potential bias between the multiplex assay and the IS, 20 representative mixed cell line RNA samples were tested with BCR/ABL1 Quant and an IS reference method (International IS Reference Laboratory, Adelaide, Australia).
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