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Exact(5)
The specificity was significantly reduced to 15~30% even with the addition of the primer blockers and increased human cot-1 DNA in the hybridization mix (data not shown).
The intensity of the actin cortices was found to be higher at lower temperatures, most likely due to the temperature-induced changes in the balance between actin assembly and disassembly dynamics in our system, also exemplified by the increased lengths of actin comet tails at 20°C compared to 30°C in the same motility mix (data not shown).
There were no statistical differences (Kruskal Wallis p = 0.94) found in the distribution of the patient groups across the three clinic years in terms of both the patient population mix (i.e., predefined patient groups) and the patient population visit mix (data not shown).
Preparations of cDNA were diluted (1 25 to 1 100) and tested by qPCR using RBCS1-specific primer pairs (Table 1) designed using Primer Express software (Applied Biosystems), which were preliminarily tested for their specificity and efficiency against a cDNA mix (data not shown).
Baseline and demographic characteristics did not differ substantially by year of diagnosis, although individual years had some small differences in the Charlson score (slightly declined over the study period), and the distributions of age, region, education levels, and treatment mix (data not shown).
Similar(55)
The extrapolation power related to phase equilibria or heat of mixing data not involved in the parameter fitting procedure of the two models were analyzed carefully.
The sequences showed a patient-specific clustering with high bootstrap support (>90%), which argues against the occurrence of contamination and sample mix-up (data not shown).
Phylogenetic analysis of V3 sequences from the entire PCR products showed distinct clusters of sequences for each patient that excluded any possibility of sample contamination or mix-up (data not shown).
The results from reactions containing FastStart mix had significantly lower average Ct values than their Excite™ 2x mix reaction counterparts (data not presented) – the difference is approximately 1 cycle per plasmid dilution.
Comparing results obtained with contrast mixes 1 and 2 (data not shown) demonstrated that contrast mix 1 was better by serving both as an MRA and a CTA agent.
However, isolation of 11A-scFvFc expressing cells could not be reliably achieved at a mixing ratio below 1∶1000 (data not shown).
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