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The RC was not stirred because the flux rates across the membrane were identical with or without stirring (data not shown).
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An activating peptide KRFK also had little or no effect on stirring-dependent TGF-β1 activation (data not shown) Since thiol-disulfide exchange contributes, at least in part, to shear-induced TGF-β1 activation[12] we tested whether the TSP-1-mediated contribution involves thiol-disulfide exchange.
The nanoparticles suspension was kept under continuous stirring at 300 rpm (RPM preoptimized, data not shown) for 3 h at 30°C to allow the complete evaporation of acetone, leaving behind the colloidal suspension of Lzp-PLGA-NPs in aqueous phase.
For retracting the particles from the evanescent field of the ATR element, the ultrasound transducer was driven around fr 1.870 MHz (at 1.6 W t.e.p.i .. Stirring speeds between 600 and 1200 rpm were tested: an increase in stirring speed led to a decrease of absorption values of the recorded spectra due to Stokes drag (data not shown).
According to the preexperiment results (data not shown), the bioreactor was operated with optimized temperature, airflow at 1.5 vvm, and stirring at 500 rpm, and the pH was uncontrolled during fermentation.
Saute briefly while stirring; do not brown.
Random is not the same as haphazard; stirring is not the same as sticking in the spoon without looking.
Stirring is not required.
When stirring, do not mix the colors.
Not a Tumblr was stirring, not even about Leo Strauss.
Cook briefly, stirring, but do not brown.
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CEO of Professional Science Editing for Scientists @ prosciediting.com