Sentence examples for minimum fold energy from inspiring English sources

The predicted minimum fold energy of 5'UTR sequences was determined using the Vienna RNAfold WebServer [ 42].

Plant sp, NSs, Nucleotide substitutions between known plant query miRNAs and the corresponding miRNA in Boechera apomictic species; NN, Number of nucleotides hairpin length; ARM, mature miRNA location in hairpin structure; AMFE, Adjusted minimum fold energy; MFEI, Minimum fold energy index; EST ID, Identifier of the 454 transcripts from which miRNA was derived.

Plant sp, NSs, Nucleotide substitutions between known plant query miRNAs and the corresponding miRNA in Boechera sexual species; NN, Number of nucleotides hairpin length; ARM, mature miRNA location in hairpin structure; AMFE, Adjusted minimum fold energy; MFEI, Minimum fold energy index; EST ID, Identifier of the 454 transcripts from which miRNA was derived.

AGO, argonaute; ESTs, expressed sequence tags; GSS, genome survey sequences; miRNA, microRNA; miRISC, miRNA-induced silencing complex; MFE, minimum fold energy; MFEI, minimal fold energy index; AMFE, adjusted minimal fold energy; PMRD, plant miRNA database; TAIR, arabidopsis information resource; SBP, squamosa promoter binding protein; qRT-PCR, quantitative reverse transcription PCR.

Briefly, a miRNA had to fulfill the following criteria to be considered in the final dataset: (1) A distinct 5′ terminus of the mapped miRNA (guide strand) and miRNA* (passenger strand), (2) the presence of a 2 nucleotide 3′ overhang of the miRNA-miRNA* duplex, and (3) a pre-miRNA fold-back structure that had a minimum fold energy (MFE) < −25 kcal mol-1[ 90].

The MFEI (Minimum Fold Energy Index) is another parameter that was used to evaluate the novel miRNAs precursors.

The minimum folding energy (MFE) of 60 nt RNA segments immediately upstream of the initiation codons was calculated as a proxy of secondary structure stability.

The minimum folding energy (MFE) was computed using DAMBE which incorporates the function library of the Vienna RNA package [22], at 37°C, with no lonely pairs and with no GU pairs at the end of helices.

We found that the IRES activity of yeast IRESs is strongly associated with weak secondary structure measured by the minimum folding energy (MFE, in kcal/mol) of the 60 nt immediately upstream of the initiation AUG (Figure 1, r = −0.7756, p = 0.0002), contrary to the conventional belief that IRESs should have complex and stable secondary structure [4] [7].

L3utr length of 3′ UTR, dG minimum folding energy.

The minimum folding energy (MFE) of the obtained 2562/1449/2475 hairpin precursors was evaluated with the UNAFold program.