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Finally, after testing these 48 transcripts, 12 could be considered as labile transcripts with a minimum fold decrease of −2.65 log10 (value arbitrary chosen), and 11 of them could be considered as persistent transcripts with a maximum fold decrease of −1.75 log10.
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VEGF-A was upregulated in 22 tumours (maximum 4.7-fold increase), in 15 tumours downregulated (minimum 3.9-fold decrease) and in 4 tumours expressed in equal amounts as in the tumour-adjacent tissues.
In our samples, miR-217 expression was significantly down-regulated across all internal controls, with a maximum −24.39-fold −24.39-foldth U6 andecreasemum −7.19-fold decrease with U6 + U91 combinandon (p < 0.01, Table 4).
It first increases to a maximum as x is increased from 0 to ca. 0.1, then experiences a 102 fold decrease to a minimum at x = ca. 0.4, where the LPEI has been protonated to its limit and the dominating anion is HPO42–.
For Picciotto vs Xu mutant analyses the minimum fold change was increased to 2.0.
In addition, we selected only those transcripts displaying a minimum fold change threshold of +1.2.
The minimum fold change threshold was defined as two-fold.
A minimum fold change of 1.5 between groups was considered.
The minimum fold change was 2.6 for total RNA and 1.8 for amplified RNA experiments.
When applying a minimum fold induction of 0.3 (log2 scale) we identified 35 proteins that were upregulated.
Transcripts with a minimum fold expression change of two were selected as differentially expressed genes.
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