Sentence examples for minimal sequencing depth from inspiring English sources

Establishing a minimal sequencing depth for required accuracy will guide cost-effective experimental design and promote the routine application of RNA-seq.

It has been difficult to establish a universal minimal sequencing depth because of differences between factors.

*** Complexity is defined as minimal sequencing depth (in million clones) at which over half of the pairs are clonal.

It is suggested that 6-fold is a minimal sequencing depth for each individual when SLAF-seq strategy is used.

In addition, we further considered sites showing a minimal sequencing depth of 3 and calculated the proportion of these with >50% base agreement among reads.

Ignoring indels, we found identical sequences in 97.8% of the sites covered at a depth of 2. Furthermore, levels of accuracy ranging from 99.97% – 100.0% were found at sequence depths equal to or greater than 3 (a total of 7,510 sites), suggesting that a strict consensus approach considering sites with minimal sequencing depth of 2 should deliver high quality mitochondrial data.

We considered all sites with mapping and base quality phred scores of ≥30, and a minimal sequence depth of 10 as high-quality base pairs (bphiqual).

We only considered high quality base pairs (bphiqual) in downstream analyses, i.e. sites with mapping and base quality phred scores of ≥ 30, and a minimal sequence depth of 10×.

For each nucleotide position, the minimal sequence coverage depth was set to 20 reads.

The minimal sequence coverage depth was set to 20 reads for each nucleotide position.

As noted above, the likelihood that a homozygous SNP in 4 of 17 murine strains represents sequence error is minimal (<1 in 10 based on sequencing depth and high Phred quality) and manual SNP assessment indicates authenticity of the homosites identified here.