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Approximately 1.8 million CpG sites with minimum sequencing depth of 10 were included in the analyses.
We used a minimum sequencing depth of 10 as the cutoff for inclusion for DNA methylation analysis.
Genomic regions with at least three CpGs covered at a minimum sequencing depth of 10 were considered to be covered.
A minimum sequencing depth of 50× was achieved and re-sequencing failed only for a single NIEHS-UFS sample.
In order to get a more unbiased estimate, we filtered SNPs by their minimum sequencing depth in each of the pools.
With two sequencing lanes, we were able to quantify ~1.8 million individual CpG sites at a minimum sequencing depth of 10.
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We inferred a robust phylogeny based on >3000 GBS loci and >1300 SNPs (with a minimum sequence depth within individuals of 10) using maximum likelihood and Bayesian inference.
By requiring a minimum sequence depth of 20 reads per sample, 94% of the successfully amplified samples were genotyped.
In order to ensure sufficient allele sampling, as well as to prevent sequencing errors from appearing to be actual variants, all four studies use or recommend a minimum sequence depth threshold, ranging from 8- to 30-fold depth of coverage.
This value can be inserted into the logistic regression model in order to predict the minimum sequence depth required to have a 50%% chance of detecting a CDR3 sequence with this abundance and other known properties.
SNPs were called using the Probabilistic variant caller in CLC Genomics Workbench using a minimum sequence depth of six, variant probability of 50.0, required presence in both forward and reverse reads, four maximum expected variants, and one standard genetic code.
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