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The topological distance (dT) was set at 2 for searching the minimal evolution tree.
However, the P. chrysosporium CYP58 homologues form a cluster separate from other fungal CYP58 proteins on the minimal evolution tree.
These seven P450s form a separate cluster from the known P450foxy proteins of other fungi (ascomycetes) on the minimal evolution tree.
However, as evident from the minimal evolution tree, the white rot CYP63 members form a separate cluster on the tree from the CYP52 family members of the ascomycetous fungi and other CYP52-related fungal P450 families.
A minimal evolution tree (WAG + Γ model) was inferred from the protein data set using the programs TREE-PUZZLE 5.0 (to calculate distances), and Fitch (for inferring the topology) from the PHYLIP V3.6a3 program package.
For the construction of the phylogenetic trees of the GH families the sequences of the family members were aligned using Muscle (version 3.8.31) [ 58] after which the alignment was used to generate a bootstrapped minimal evolution tree (1000 bootstraps) using MEGA4 [ 59].
Similar(53)
The alternative methods of phylogenetic tree construction, minimum evolution and maximum likelihood, were tested, but the support for the minimum evolution tree was not sufficient (data not shown).
The minimal evolution trees were generated by heuristic search using the Close-Neighbor-Interchange (CNI) algorithm, with the Neighbor-Joining tree serving as the temporary tree.
Additional trees were built, using alternate phylogenetic methods (neighbour-joining trees under various models of evolution, minimum evolution trees and maximum parsimony trees) to verify the observed phylogeny.
Minimum evolution trees were generated using the program Mega2 [ 26].
What tree topologies is neighbor-joining likely to pick when it fails to construct the balanced minimum evolution tree?
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