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A Minimum Evolution tree was generated by the Neighbor-Joining method with level 1 Close-Neighbor-Interchange.
The alternative methods of phylogenetic tree construction, minimum evolution and maximum likelihood, were tested, but the support for the minimum evolution tree was not sufficient (data not shown).
Figure 1a shows a minimum evolution tree of the 40 post-vaccination era isolates.
The minimum evolution tree was calculated by the implementation of PAUP software in the GCG package.
This model was used for the minimum evolution tree based on the first two codon positions.
The D. melanogaster minimum evolution tree indicates that the Spn28F, Spn38F, Spn28B, Spn42D, Spn28Db and Spn28Da genes are related.
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The Minimum Evolution trees were calculated using complete deletion, all codon positions and a Kimura 2-parameter model, including transitions and transversions.
Phylogenies were inferred using both neighbor-joining and minimum evolution tree-building algorithms under the following constraints ((((primates), rodents), (artiodactyls, carnivores)), platypus).
Minimum evolution trees were generated using the program Mega2 [ 26].
See [ 21] for an example of a local search approach to finding minimum evolution trees.
The robustness of the minimum evolution trees was evaluated by bootstrapping with 1000 repetitions.
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