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Therefore, the optimal accumulation time of 6 min was employed in further experiments.

Ninety-six college students participated in this study, in which a game-based experiment lasting 90 min was employed.

Based on these test results, an annealing treatment with 420 °C annealing temperature for 10 min was employed.

The co-solvent mixture was also composed of CHCl3/heptane (35/65 v/v) and a gradient from 15 to 60% of co-solvent in 2.2 min was employed.

To further improve the properties, particularly the creep resistance and ductility at high temperatures, a post-solutionizing heat-treatment method that involves an intermediated annealing either at 700 or 800 °C after solutionizing for durations up to 180 min was employed.

2) Activity-dependent slowing: A train of pulses (2 Hz for 3 min) was employed to investigate activity-dependent slowing of conduction.

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Different oxidation times namely 1, 3, 5, 10, 20 and 30 min were employed and the coated samples were subjected to coating thickness and surface roughness measurements.

Three preceding episodes of bilateral carotid artery occlusion for 1 min and reperfusion of 1 min were employed to elicit ischemic preconditioning of brain, while three episodes of bilateral carotid artery occlusion for 10 s and reperfusion of 10 s immediately after the completion of were employed to elicit ischemic postconditioning of brain.

As presented in Figure 3, the fluorescence intensity increased over time for up to 3 min, then remained constant indicating that 3 min were needed to derivatize thiols in the wells and that the fluorescence intensity is stable for at least 15 min. On the basis of the results, 10 min were employed for cellular thiol derivatization for the high-throughput assay.

A 50 min gradient was employed, which consisted of a 35 min gradient from 2 40% acetonitrile with 0.1% (v/v) formic acid, after which, the acetonitrile increased to a maximum 80% for the remainder of the run.

This 2 min ITI was employed to control for potential motivational disturbances and verify that all groups made more visits to the novel arm when mnemonic demand was minimal [17].

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