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For STAT3 and pSTAT3 immunostaining, permeabilisation in cold methanol (−20 °C, 10 min) was performed.
Flow rate calibration (300 nL to 6.12 μL per min) was performed, and an accuracy >99.94% was obtained.
Treadmill exercise (20 min) was performed under two conditions: (a) self-selected speed and (b) imposed speed, which exceeded the self-selected level by a minimal amount (just 10%).
For attenuation correction, a transmission scan (5 min) was performed using a 137Cs point source.
An optional extra-cranial static PET for 10 min was performed in some of the patients.
Rinsing with a water solution for 5 min was performed between each concentration change.
A rinsing step in water for 1 min was performed in between the two polyelectrolyte baths.
A set of 10 measurements with interval of 2 min was performed at each pump power.
A short screening decontamination of 5 min was performed as recommended.
HRTEM analysis of nanowires obtained at 800 W and 5 min was performed in order to determine its structure.
In order to remove the contaminants from the ZnO target, pre-sputtering for 10 min was performed.
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