Sentence examples for min the substrate from inspiring English sources

After a pre-incubation at 60°C for 2 min, the substrate was added to the mixture and the reduction of NAD+ was monitored at 340 nm.

For Sample 3, three droplets of solution of sample 1 were dropped on the substrate in hexane atmosphere, and then after 1 min the substrate was rotated at 400 rpm.

At a deposition time of 20 to 30 min, the substrate surface becomes fully covered, and any further increase of the deposition time does not increase any more the number of the surface nanoparticles on which SERS-active (R6G) molecules can be anchored.

After pre-incubating the POP with 20 eq. of inhibitor during 15 min, the substrate was added and incubated for 30 min at 37°C.

After 15 min, the substrate was rinsed with 1 mM NaCl (10×) and subsequently with PBS (10×).

After 30 min, the substrate 2 (8.3 g, 30.2 mmol) was added and dissolved followed by the addition of NaOH (7.3 g, 181.4 mmol).

After annealing at 140 °C for 15 min, the substrates were transferred into a nitrogen-filled glove box.

After accomplishing the anodization process within 20 min, the substrates were rinsed thoroughly with DI and dried in an oven at about 658°C for 30 min.

2HBP eluted at tR=11.1 min, and the product 2,3-dihydroxybiphenyl eluted at tR=10.0 min. The substrate and product concentrations were calculated from calibration curves obtained under the same conditions.

Transfer samples, phosphatase assay buffer, substrate in a radioisotope-protective rack into the anaerobic chamber Add 0.02> ml samples containing 30 μg proteins to 1.5 ml centrifuge tubes Add 0.02 ml of phosphatase assay buffer Preincubate at 30°C for 5 min Add the substrate (>10,000 dpm) For cell lysates, incubate at 30°C for 10 min to 2 hours inside the chamber.

Si (111) substrates are deoxidized before growth in a 10% HF solution for 1 min. The substrate surface is then cleaned and smoothed with a 20-min bake at 1,100°C and 100 mbar under H2.