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The time difference between UAV5D and B2 measurements was ~ 40 min at the top and ~ 3 min at the bottom (Fig. 1b).
Next, the discs were exposed to intense light measuring 800 μmol m-2 s-1 for 30 min at room temperature, and air was supplied for 10 s every 10 min from the bottom of containers during intense light treatment.
Samples were then centrifuged at 3000 rpm for 5 min. The bottom layer was removed to a round bottom flask.
In our work, for short duration dives (37 ± 16 min at the bottom) performed by healthy subjects, hyperoxia did not contribute to the increase in ULCs.
A two-phase system (top: aqueous, bottom: organic) was generated after shaking for 1 h and centrifugation at 3000 rpm at room temperature for 5 min. The bottom organic phase was collected and concentrated under a stream of nitrogen at 55°C giving a residue that was resuspended in 600 μL of hexane.
The liquid fraction was transferred to a separating funnel and allowed to settle for 30 min. The bottom layer consisting of methanol, residual catalyst and soluble sub-millimeter particles was removed, and the upper layer consisting of the biodiesel produced and n-hexane was washed several times with warm (~50°C) de-ionized water.
A two-phase system (top: aqueous, bottom: organic) was generated after shaking for 1 h and centrifugation at 8000 rpm at room temperature for 15 min. The bottom organic phase was transferred to a new glass tube and evaporated to dryness under a stream of nitrogen at 55°C.
The same was then centrifuged at 1000 rpm for 5 min and the bottom layer recovered and dried.
This fraction was then centrifuged at 240 g for 5 min and the bottom quarter fraction was collected [ 16].
After 48 h, dextran-FITC was added to the upper chamber and was recovered after 40 min from the bottom chamber and measured in a fluorimeter.
Fluorescence readings were taken at 480 nm every 15 min from the bottom of the wells after excitation with 20 flashes per well at 450 nm.
