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All animals were acclimatized to the test chamber 30 min before testing.
It should be pointed out that all samples are sonicated for 20 min before testing.
They were transferred to a bioassay room, and allowed to acclimate for at least 30 min, before testing.
Escalating doses of cortisol (0, 3, 6, 12, 24 mg) were administered 8 min before testing according to a between-subjects design.
It had no effect when administered 30 min before each conditioning session during CPP training period (acquisition) or 30 min before testing for place preference in the absence of morphine (expression).
Mice were brought to the laboratory 60 min before testing between 9.00 AM and 6.00 PM.
The mice were then placed into their home cages for 10 min before testing for short-term memory.
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Between tests in solutions expected to activate currents, the cilium was kept in an appropriate control bath for 2 min. Before testing for effects of ITP or ATP, the cilium was kept in a control bath containing the same nucleotide.
Serum samples were heated for 30 min at 56°C before testing.
All sera were heat-inactivated for 30 min at 56°C before testing.
Serum samples were heat-inactivated for 30 min at 56°C before testing.
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