Sentence examples for min a final from inspiring English sources

The thermal cycling conditions included: a reverse transcription step at 48°C for 30 min, an inactivation step of RT/RNAse enzyme at 95°C for 10 min followed by 40 cycles of 95°C 15 s, 60°C 1 min, a final denaturation step where the temperature increases from 60°C to 95°C during 20 min and a step of 15 sec at 95°C.

At t = 240 min the kilocalories consumed were counted and at t = 290 min a final blood draw was performed.

PCR parameters were: initial denaturation at 94°C for 2 mins, followed by 30 cycles of 94°C for 30s, 50°C for 30s and 72°C for 1 min, a final extension of 72°C for 5 min.

Reaction conditions were 75°C, 3 min; 94°C, 4 min; 33 cycles of 94°C, 1 min; 49°C, 1 min; 72°C 2 min; a final extension of 72°C for 7 minutes.

Thermocycling parameters were 94°C for 10 min, 35 cycles of 94°C for 30 s, 55°C for 1 min, and 72°C for 2 min; a final extension step at 72°C was added for 5 min.

The amplification condition was 50°C for 2 min; 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 59°C for 1 min; a final soak at 4°C was also incorporated.

Each reaction included an initial 5-min denaturation at 94°C, followed by 21 to 38 cycles of PCR (94°C, 45 min; 60°C, 45 min; 72°C, 1 min), and a final 10 min at 72°C.

The conditions for amplification of hptII were 94 °C/5 min, 28 cycles of 94 °C/1 min, 65 °C/1 min and 72 °C/1 min, and a final extension at 72 °C/5 min.

PCR was performed at 95°C/4 min, followed by 35 cycles of 95°C/1 min, 60°C/1 min, and 72°C/1 min, with a final extension of 72°C/60 min.

The PCR conditions are as follows: 94°C for 3 min, followed by 35 cycles of 94°C for 1 min, 59°C for 1 min, 72°C for 5 min, with a final 10 min extention at 72°C.

Subsequently, 33 additional cycles were conducted with the following program: 92°C for 1 min, 56°C for 2 min, 72°C for 2 min and a final 5 min extension step at 72°C.