They behave as NAD mimics with the 5′,5'-diphosphate group substituted by a 8,5′ thioglycolic bridge.
Two-trait mimics with the same colour and pattern or the same colour and shape as their former models were avoided as frequently as perfect mimics, while colour-only mimics were avoided more frequently than shape-only mimics.
Classical tissue engineering is based on culturing cells in scaffolds, artificial extracellular matrix mimics, with the hope that the process leads to tissues and organs, that upon implantation can replace damaged, dysfunctional ones and lead to regeneration.
Here, we redesign them to encode minimal primary miRNA mimics, with the long term aim of intracellular transcription followed by RNA processing and maturation via endogenous pathways.
The miRNA mimics from Applied Biosystems impacted ABT888 sensitivity over a broad range, ∼10- to 12-fold difference between the mimics with the highest impact on viability and the control mimics.
By cotransfecting the miRNA mimics with the corresponding constructs, we found that all three miRNAs significantly repressed luciferase activity compared with miR-negative control (NC) (scrambled oligonucleotides) and mock (water) (Fig. 4A and B).
