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As shown in Figure 2, in all cases we observed that P E increases from a low value in young duplicates with the increasing of duplication ages; this P E -age (t) correlation is statistically significant (P < 10−4, χ-test).
All conventional RT-PCR (HAV, SH-Prot and SH-Poly systems) and TaqMan (SH-Prot, SH-Poly, JN and SH-5U systems) assays evaluated were consistent for the detection of the three different HAV strains (HM-175, HAS-15 and LSH/S) used and reproducible for both RNA duplicates with the exception of two reproducibility discrepancies observed with both 5′-UTR real-time systems (JN and SH-5U).
Shake flask fermentation was conducted in duplicates with the hydrolysates (Table 2) under same conditions as previously described.
After cross checking for duplicates with the 13 articles that were already eligible, 12 studies were added, so a total of 25 studies were eligible for methodological assessment.
All reactions were run in duplicates with the mean quantity taken.
Real-time qPCR was performed in duplicates with the Light Cycler480 instrument (Roche Diagnostics) using the Roche's SYBR-Green mastermix and prevalidated primer pairs (Qiagen).
Similar(26)
I imagine someone going into my copy and hand-removing the duplicates with exasperation, the way I comb the house collecting my kids' empty Vitamin Water bottles.
Results from the CMAS/TBC interaction experiments could duplicate with the damage patterns on the real combustion chamber parts.
All the experiments were performed in duplicate, with the average value reported.
The spectral evolution was completely duplicated with the irradiation of anthracene embedded in KCl or NaCl.
Samples were set up in duplicate with the Power SYBR® Green and analyzed with the ABI 7500 Real-Time PCR System (Applied Biosystems, Life Technologies Corp., Carlsbad, CA, USA).
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