The data presented are the mean values of three replicates with the standard deviations.
All experiments were done with a minimum of two replicates, with the mean value considered for further analysis.
Values are mean and standard deviation (SD) obtained from the replicates, with the latter shown in parentheses.
Of these, blackwood, alder and radiata had three surviving replicates with the other species having only two each.
The design was an RCBD with a split-plot arrangement and three replicates, with the crops as the main plots, and N rates as the subplots.
For checking the incongruence between plastid and nuclear data, the incongruence length difference (ILD) test (Farris et al. 1994) was performed in PAUP* version 4.0b10, and 1000 replicates with the same settings as in the heuristic searches were conducted.
Branch support was estimated using 1000 bootstrap replicates with the same heuristic settings.
During latent infection, the viral episome is tethered to the host chromosomes and replicates with the host cell.
Bootstrap support (BS) was assessed with 1,000 replicates with the rapid bootstrap algorithm implemented in RAxML [31].
A phylogenetic tree using amino acid sequences of LKR/SDH from different sources by the neighbor-joining method verified the confidence of the branching order by 1,000 bootstrap replicates with the MEGA 4.0 software.
Each treatment was replicated 27 times, comprising nine spatial replicates sampled over three temporal replicates, with the second and third temporal groups defoliated approximately 3 and 7 days after the first temporal group, respectively (see Figure S1 for exact dates).
