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Here, we analyzed the role of Ca2+ signaling in immature DC migration in confinement.
We here unraveled a new role for intracellular Ca2+ dynamics in the regulation of amoeboid-like cell migration in confinement.
Whether and how velocity fluctuations are regulated during DC migration in confinement is the aim of the present study.
We propose that by upholding myosin II activity, constitutive calcium release from the ER through IP3R1 maintains DC polarity during migration in confinement, facilitating the exploration of their environment.
In a recent study, we combined theory and experiments to investigate the origin and magnitude of the forces involved in the migration of Walker carcinosarcoma cells, which do not rely on specific adhesions and display active migration in confinement [ 34].
We further identified the mechanism involved by showing that inhibition of intracellular Ca2+ release by silencing IP3R1 compromised the activity and intracellular distribution of myosin IIA, the main force-generating protein involved in fast DC migration in confinement.
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Together, these data suggest that the regulation of cortical contractility might serve as a universal switch for transforming cells into an amoeboid cell migration mode characterized by fast and highly directional migration in 3D confinement.
Our results identify a mechanism by which Eps8 may promote the transition to rapid, unregulated migration of cancer cells in confinement that may be critical to their highly invasive behavior in vivo.
Citing evidence from observations in humans, work in experimental animals, and inferences from related studies in other fields, the authors note that a "wealth of ecological associations link TB with malnutrition in populations affected by famine, war, natural disasters, poverty, mass migration, and confinement in prisons or ghettos".
Immigration officials also say the detention policy that is keeping all the Haitians in confinement is intended to prevent future mass migrations.
He was kept in confinement at Cucusus in Armenia.
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