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Exact(12)
The affinity-purified recombinant enzyme, when analyzed by sodium dodecyl sulphate –polyacrylamide gel electrophoresis (SDS PAGE), migrated as single band of an expected size.
It was observed that the V1G mutant migrated as a doublet (Figure 4A), whereas other mutants as well as the R-BSX migrated as single bands when samples were incubated at 25°C.
It was demonstrated that linker with 5 amino acid residues was sufficient to separated HSA and IFN-α2b effectively, as fusion protein with this linker migrated as single band on non-reducing SDS PAGE.
It is notable that whereas non-expressing cells (Tet−) collectively moved, scapinin-expressing cells (Tet+) migrated as single cells (Figure 4D .The parental Hela cells used in this study moved in sheet-like structures like the non-expressing cells.
The migration speed of control cells was determined from cells localised within cell colonies (Fig. 2C), but some cells in the population migrated as single cells (Movies S1 and S3).
The purified proteins migrated as single bands, and the apparent molecular weights were in agreement with the predicted molecular masses for the constructed proteins 18483.61 for CssA (with the polyhistidine tag) and 43,997.93 for CssB (fused to glutathione-S-transferase carrying a His tag (Fig. 1).
Similar(48)
The His tagged-Hp0310 was eluted as a single peak, roughly corresponding to a tetramer and migrated as a single 37.5 kDa species on SDS-PAGE (theoretical mass: 33.7 Kda plus the 2.8 Kda His-tag).
The wild-type and mutant proteins were expressed, purified, and migrated as a single band at an apparent molecular mass of ~ 60 kDa on SDS-PAGE (Additional file 2: Figure S1).
The purified CD81 migrated as a single band at around 27kDa on 16% SDS-PAGE (Fig. 2A).
During electrophoresis under non-denaturing conditions, apoHmuY migrated as a single band with an Rf greater than that of the α and β haemoglobin chains (Fig. 9).
ATF1, however, migrated as a single band of 35 kDa on SDS-PAGE gels that was not affected by IR (Fig. 1B).
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