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Although the in vivo pattern of endogenous astrocyte migration following CNS injury is unknown, glial cells migrate as single cells during development (Klambt, 2009).
These are traditionally thought to migrate as single cells in a fibroblast-like fashion.
Gliomas are known for their diffusely infiltrative invasion pattern, in which the tumor cells migrate as single cells.
Both linkers and staples migrate as single bands on SDS-PAGE gels (NuPAGE 10% Bis-Tris Gel, Invitrogen) (Supporting Information Figure 1A).
When injected singly, IsdBN1 and metHb migrate as single species with apparent molecular mass of 19.4 kDa and 32.5 kDa, consistent with monomeric and dimeric forms, respectively.
Epithelial mesenchymal transition (EMT) is the directional process where sessile, polarised epithelial cells alter the expression of key adhesion and regulatory molecules and gain the ability to survive and migrate as single cells.
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While cancer cells migrating as single cells can employ either the protease-dependent mesenchymal migration or protease-independent amoeboid-like migration, collective cell migration employs only mesenchymal cell migration.
It was demonstrated that linker with 5 amino acid residues was sufficient to separated HSA and IFN-α2b effectively, as fusion protein with this linker migrated as single band on non-reducing SDS PAGE.
It was observed that the V1G mutant migrated as a doublet (Figure 4A), whereas other mutants as well as the R-BSX migrated as single bands when samples were incubated at 25°C.
It is notable that whereas non-expressing cells (Tet−) collectively moved, scapinin-expressing cells (Tet+) migrated as single cells (Figure 4D .The parental Hela cells used in this study moved in sheet-like structures like the non-expressing cells.
The migration speed of control cells was determined from cells localised within cell colonies (Fig. 2C), but some cells in the population migrated as single cells (Movies S1 and S3).
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