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Additionally, the putative esterase (LipO, spot 10) migrated as "free" proteins because of no attachment to other protein subunits using STRING database analysis.
Thus, the protein migrated as "free" proteins in 2-D gel.
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As previously observed [ 33], the monomeric form of exogenous Ero1β-WT migrated as two distinct redox species (Red and OX) when cells were treated with NEM to alkylate free thiols in situ prior to lysis.
Purity of CBH I was confirmed by SDS PAGE, where isolated enzyme migrated as single Coomassie-stained protein band.
The 6.8 kDa rCCK-58 and 18.7 kDa rfhSP-D migrated as expected on SDS-PAGE (Fig. 4g,h).
When encapsulated, VICs attained spread morphology, and proliferated and migrated as shown through real-time cell microscopy.
Under reducing conditions, where the disulfide bonds of the IgG1 Fc hinge region are disrupted, aflibercept migrated, as expected, as a major band of ~70 kDa.
Interestingly, shR9 bound RIG-I migrated as two distinct bands.
DOR migrated as a 40 kDa protein in SDS-PAGE (Figure 2B).
The purified CD81 migrated as a single band at around 27kDa on 16% SDS-PAGE (Fig. 2A).
The AMPA receptors containing subunits 2/3 migrated as two distinct high-molecular-weight complexes.
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