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LOH in tumor DNA relative to nonmalignant lung DNA was determined at 52 microsatellites located on ten chromosomal loci.
As microsatellites located on Y chromosome mutate with different rates, they may be exploited in evolutionary studies, genealogical testing of a variety of populations and even, as proven recently, aid individual identification.
The following seven microsatellites located on Xq21 were also analyzed in all samples: DXS995, DXS8076, DXS1012, DXS1002, DXS1019, DXS8114 and DXS1050.
The 11,623 putative microsatellites located on unigenes obtained from Sodom apple provided very helpful resources for designing primers (Additional file 4) which will be used for developing molecular markers and serving various research purposes in Sodom apple.
None of the genes that were located in the linkage group matching SSC 12 have previously been mapped in pig and hence the verification and orientation of this chromosome relies on the comparative map and linkage to microsatellites located on SSC 12.
Similar(54)
In our previous studies [ 39, 47], we identified significant QTL for growth traits on LGs (linkage groups) 2 and 3. To map the QTL on LGs 2 and 3 with more precision, 33 additional microsatellites located near the QTL on these two linkage groups were genotyped.
LOH analysis was performed (only for fresh samples) based on polymorphic microsatellites located close to putative tongue cancer tumor suppressor genes including tp53CA (TP53-17pl3.1), D3S1300 FHIT-3p14.2 FHIT-3p14.248 (CDKN2andp21).
Additional 33 novel microsatellites located near QTL for growth traits on LGs 2 and 3 were selected and mapped with QTL panel containing 380 offspring.
Analysis was performed on 3 Pfdhps-linked microsatellites located 0.8 kb (marker [m.] 0.8), 4.3 kb (m. 4.3), and 7.7 kb (m. 7.7) downstream of the coding position 437 of Pfdhps, located on chromosome 8 (34 ).
Some 69% of the microsatellites were located on scaffolds assigned to the chromosomes based on the 8.4 X assembly, and the position of the remainder is unknown (See additional file 2).
The number of markers on the linkage map of L. calcarifer increased to 302 by mapping 62 novel microsatellites located in 62 BAC clones onto the map.
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