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We have analysed by polymerase chain reaction (PCR) assay replication errors (RER) and loss of heterozygosity (LOH) at microsatellites mapped on chromosomes 2p and 3p in 64 paired tumour-normal DNA samples from consecutively resected stage I, II or IIIA NSCLC.
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All linked microsatellites mapped consistently on the same linkage groups in the two parental maps and 82% of the markers mapped with the same order along the homologous parental linkage groups with most order changes concentrated on linkage groups 1, 8, 9 and 10.
The remaining 306 microsatellites were mapped on the B genome component of the tetraploid map.
Although 70 microsatellites were mapped on a framework map of RAPD markers [ 15], 34 on a framework of AFLP markers [ 30], and 40 on a combined RFLP and candidate gene map [ 17], the genus Eucalyptus still lacks a more comprehensive genetic map built exclusively with microsatellite markers.
EMBRA62, remained unlinked to the map constructed in this study, possibly due to the very low density of microsatellite markers mapped on group 11 for E. urophylla.
Segregation data sets of RFLP, AFLP and microsatellite markers mapped on previous red clover maps were used for the construction of the consensus map (Table 1) 1, 2, 3. Markers designated with a single 'C' and a number indicate RFLP markers, while 'C_PK_' and 'V_PK_' followed by a number represent AFLP markers.
A total of 21 294 primer pairs were designed, of which 15 573 microsatellites were physically mapped on 9 chromosomes of foxtail millet genome, and 159 markers were validated successfully in foxtail millet accessions.
Without exceptions, all microsatellite markers that mapped on the same linkage group in one species also did so in the other species.
A total of 2,099 BAC end sequence-derived microsatellite markers were mapped on the catfish linkage map ([ 48], Table 2).
Foxtail millet rice synteny: the microsatellite markers physically mapped on the foxtail millet chromosomes showed least synteny with 12 rice chromosomes with average frequency of 6.4% that is much lower than that with the sorghum and maize chromosomes (Table 3; Fig. 5C).
For DArT, microsatellite (SSR) and gene markers mapped on each component map, the percentage of markers unique to that map (i.e. not mapped in any of the six other component maps) are given in parentheses.
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