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pylori microbial sequences were obtained.

After identification and removal of human reads, microbial sequences were quality filtered and trimmed.

With the development of high-throughput and low-cost sequencing technology, a large number of marine microbial sequences were generated.

For example, in three separate studies 3 Gb of microbial sequences were generated from fecal samples of only 33 individuals from the USA or Japan [ 2, 29, 37].

Interestingly, microbial sequences were also recovered from sterile buffer washes of bronchoscopes: although "microbiologically sterile", nucleic acid fragments could persist through manufacturing or sterilization processes.

Non-human RNA sequences were subjected to a microbial discovery bioinformatics platform, and microbial sequences were analyzed by Metastats for association with KD.

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Candidate microbial sequences are summarized in Table 6.

Recognition of these microbial sequences is mediated by a member of the TLR family, namely TLR9.

As expected, although a minority of microbial sequences is present, >85% of the sequences generated from the termite gut tissue are homologous to known genes previously sequenced from insects or from other invertebrates.

The idea of computational subtraction of human host sequences to identify microbial sequences was first implemented on Amazon EC2 (Elastic Compute Cloud) environment in the form of software PathSeq (Kostic et al., 2011).

The scarcity of long k-mers, together with the fact that most microbial sequence is non-redundant, has the consequence that most k-mers that do occur are present only once, contain sequence from single copy genes, and map to unique locations of the genome.

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