Bisabolane is generated by hydrogenation of (Z -α-bisabolene, which in turn is generated by the three-domain plant sesquiterpene cyclase, α-bisabolene synthase from Abies grandis, in a microbial expression system.
Despite advances in microbial protein expression systems, low production of proteins remains a great concern for some genes.
Although lipases are known to occur in a diversity of organisms, including animals, plants and microbes, the current industrial production of these enzymes relies almost exclusively on microbial-based expression systems.
As an emerging subdivision of the biopharmaceutical industry, PMF is still trying to gain comparable social acceptance as the already established production systems that produce these high valued proteins in microbial, yeast, or mammalian expression systems.
Smaller proteins are supposed to be produced more economically in microbial cells, whereas mammalian expression systems are exclusively used for large glycosylated proteins.
We will also provide readers with some general information on transport phenomena in stirred bioreactors before discussing special design aspects and performance parameters for expression systems (mammalian and microbial cells) that prevail in commercial biopharmaceutical production processes.
