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However, our RNA-Seq based transcriptome profiling indicated that the ICU and CC distribution patterns of different gene expression classes in CHO cell are relatively similar, unlike other microbial expression hosts, Escherichia coli and Saccharomyces cerevisiae.
In addition to providing the molecular machinery for transcription and translation, recombinant microbial expression hosts maintain the critical genotype-phenotype link that is essential for high throughput screening and recovery of proteins encoded by plasmid libraries.
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Specifically, publicly available genomic, transcriptomic, proteomic and translatomic data for microbial and mammalian expression hosts, Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris and Chinese hamster ovary (CHO) cells, were compiled to derive their individual codon and codon pair frequencies.
As the majority of natural microorganisms are not cultivable under laboratory conditions, the application of robust microbial hosts for heterologous expression of genes and gene clusters is crucial both to understand microbial genetic diversity as well as to develop new value-added products.
Expression of enzymes in microbial hosts offers the possibility to eliminate the risk of the biological material variability within the population of differing, diploid individuals, sometimes at different life cycle stages.
Through novel implementations of ICO, CCO and MOCO, the high-expression genes of four microbial hosts were optimized and cross-validated to compare the performance of the optimized sequences.
Despite relatively small effects on mediating microbial-induced host gene expression changes, MyD88 affects host microbial ecology in the gut.
The links between microbial infection, host cell metabolism and gene expression regulation have been widely documented in the case of viruses.
These findings have uncovered a unique example of microbial regulation of host gene expression that retards cell cycling and inhibits colon cancer cell proliferation.
These findings have uncovered a unique mechanism of microbial interaction with host gene expression that involves alterations of miRNA profiles to restrain cell cycling and inhibit colon cancer cell proliferation.
The aim of this study was to present a new approach that combines RNA microbial identification with host gene expression to characterize and validate metagenomic taxonomic profiling in individuals with asthma.
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