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Microarray fluorescence intensities (the average of 5 biological replicates - log2) and qRT-PCR Ct values (averages of 3 technical replicates) were plotted in Prism 5 for Mac OSX and analyzed by a Spearman Rank Correlation test.
However, no transcriptional differences were observed in B. anthracis for Sap, and both microarray fluorescence intensities and transcriptome sequencing data suggest that this gene is constitutively expressed at a substantial level in both conditions (Passalacqua et al., unpublished).
Microarray fluorescence intensities were extracted using BeadStudio 2.0 (Illumina).
Microarray fluorescence intensities were downloaded from Gene Expression Omnibus (GEO) using accession number GSE41633.
In the two-dye microarray, fluorescence intensities of two samples, each labeled with a different dye, are compared after hybridization.
We compared the absolute levels of gene expression in terms of RNA-seq counts and microarray fluorescence intensities for all the listed genes called by both the methods.
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Four wheat and four barley sequences where the overall microarray fluorescence intensity in wheat is significantly higher than in barley are indicated by "W".
Expression data for barley (Panel A, Figure 5) show that these sequences exhibit a microarray fluorescence intensity that is significantly at odds with that expected from the corresponding QPCR signal.
Low, moderate and high expression values are calculated on the basis of the microarray clones fluorescence intensity and correspond respectively to values lower than 3,000, comprise between 3,000 and 10,000 and higher than 10,000 arbitrary fluorescence units.
For the determination of the global A. gambiae female salivary gland transcriptome, the microarray spot hybridization fluorescence intensities were used as an indicator of transcript abundance/expression level, since each spot contained a similar amount of nucleic acid.
Images of the microarray hybridizations were acquired using the Agilent G2565AA microarray scanner and the fluorescence intensities were obtained with QuantArray v3.0 software (PerkinElmer).
Related(12)
microarray fluorescence intensity
microarray hybridisation intensities
microarray channel intensities
microarray expression intensities
microarray log2 intensities
microarray fluorescence data
microarray fluorescence signals
microarray probe intensities
microarray spot intensities
microarray fluorescence measurements
microarray hybridization intensities
microarray signal intensities
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