Sentence examples for microarray fluorescence measurements from inspiring English sources
Microarray fluorescence measurements were acquired as test (Hin6I-digested DNA labeled with Cy3) versus control hybridization intensity (mock-digested DNA labeled with Cy5).
The data presented here demonstrate the effects of both ozone and humidity on microarray fluorescence measurements for both in-house fabricated arrays and those manufactured by Agilent Technologies (Palo Alto, CA, USA), and the dramatic improvement in fluorescent dye stability under the newly implemented carbon filtration system.
Fluorescence measurements on protein microarrays demand photodetectors with ultra-high sensitivity (down to the single photon level), low noise and parallel readout capability.
Upon hybridization, microarrays were washed and dried according to the Agilent's instructions and fluorescence measurements were performed using an Agilent Technologies G2565B microarray scanner.
Fluorescence measurements were normalized over all 18 microarray chips.
The results of gene expression analysis (microarrays and quantitative real-time PCR) as well as gas exchange and fluorescence measurements in PVYNTN-infected samples were normalized to the average values measured in mock-inoculated samples at each time point.
Upon hybridization, microarrays were washed and dried according to the Genisphere's instructions and fluorescence measurements were performed using a ScanArrayLite (Perkin Elmer) laser scanner.
To assess clinical samples using the cellular microarray, particularly for comparative purposes, a standardized data analysis system is required to transform original fluorescence measurements into normalized results suitable for inter-/intra-assay comparison.
For each microarray, we started with the NimbleGen-supplied file of normalized log2 ratios of sample:reference fluorescence measurements at every probe location.
Fluorescence measurements were performed using an FP-8500 spectrofluorometer.
P.M and P.C performed the thioflavin-T fluorescence measurements.
