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The resulting microarray signal intensities for all 12,488 probe sets were normalized and the background was subtracted.
The results of the current study showed similar levels for both microarray signal intensities and transcript expression of the five housekeeping genes tested (arcA, gapA, mdh, rfbA, and rpoS) following EHEC growth in the presence and absence of epithelial cells.
Microarray signal intensities were normalized using the gcrma package [ 19].
Microarray signal intensities were determined using BRB-ArrayTools (http://linus.nci.nih.gov/BRB-ArrayTools/).
The microarray signal intensities from two biological replicates were plotted on X-Y scatter plots.
Respective microarray signal intensities of labeled NASBA products are shown on Fig. 1B.
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After fully correcting the subtype from the result of microarray signal intensity, the microarray output method combined with bioinformatics tools, identified and monitored genetic variations of H5N1.
The microarray signal intensity data from all four alleles (A, C, G, and T), on both the sense and antisense strands formed the relative expected allele intensity.
Visual inspection of residual sign images of.cel files using Affy PLM [33] revealed no major geographic defects in microarray signal intensity.
Mean DNA microarray signal intensity of total detected genes was significantly greater in organically managed soils than in conventionally managed soils (Table 7) [42].
Microarray signal intensity normalization has been widely used to adjust for experimental artifacts between the samples.
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