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This review summarizes the major microarray findings of relevance to neuropsychopharmacology, as a prelude to the design and analysis of future basic and clinical microarray experiments.
This review highlights preclinical microarray findings of the ischemic brain, discusses the transcriptome of cerebral preconditioning and emphasizes the importance of further characterizing the role of the neurovascular unit and peripheral white blood cells in mediating stroke damage and repair within the penumbra.
Expression of miR-342 in the larger cohort of breast tumours (n = 95) using RQ-PCR confirmed the microarray findings of an association between miR-342 and ER positivity.
To confirm the microarray findings, of above 21 differentially expressed miRNAs, 5 (miR-375, miR-424, miR-181b, miR-224, and miR-27a) were selected and validated in paclitaxel-treated cervical cancer cell lines (Caski and SiHa).
We next evaluated the cell cycle phases in U87 and A172 cells in order to corroborate the microarray findings of differentially expressed genes in pathways related to cell cycle regulation.
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After analysis of the microarray findings on TMeV 4.6.0 software [ 39, 40], one-way ANOVA results of the 4 groups uninfected wild, uninfected offshore, low and high infected (respectively CRIC, CAB, BL and i) having a p value < 0.01 with Bonferroni's correction found that 249 of 31,918 analyzed ESTs seemed to be differentially expressed.
The focus of the discussion and conclusions should be on the microarray findings on proteases and the wealth of functional data on proteases presented in the manuscript.
Moreover, increased COMP and TGFβR3 transcript abundance for adult cartilage was also validated by RT-qPCR while TGFβ1 confirmed the microarray finding of no significant difference.
QPCR confirmed the directionality of the microarray findings for nine out of the twelve genes tested (75%).
Expression of five of those genes was measured using RT-qPCR (Additional file 2: Figure S2), and the results for each gene were consistent with the microarray findings for the effect of 4× and day of lactation on mammary gene expression.
In addition, several genes detected by microarray analysis (i.e. CXCL1, IL-1β) have previously been shown to be down-regulated in brain abscesses of MyD88 KO mice at the protein level (Kielian et al., 2007), confirming the validity of the microarray findings to accurately distinguish changes in gene expression between the groups.
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