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The mice were thereafter daily orally treated over a 19 or 22-day period with 0, 38, 75, 2 × 75 mg/kg body weight (b.w).

The mice were thereafter humanely killed before recovery from the anaesthesia.

The mice were thereafter imaged biweekly for another 16 weeks up to 22 weeks of age.

Scn1afl/+ mice were thereafter maintained by crossing with C57BL/6J mice.

The mice were thereafter housed with free access to water and food under specific pathogen-free conditions.

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Each of the test mice was thereafter treated with either distilled water (DW), Diclofenac sodium (10 mg/kg BW) or ethanol extract of C. zedoaria at the doses of 250 and 500 mg/kg BW orally.

Human, mouse and rat sequences were thereafter multiply aligned, and used to further search the human genomic area (chrX:99850000 100250000) using an HMM [ 51].

Thereafter, mice were returned to their new testing cage.

Thereafter, mice were monitored at least twice a week for wellness and the development of palpable tumors.

Thereafter mice were sacrificed and tumors were excised, dried (60°C, 16 hr), and weighed before Evans blue extraction in 1 ml of formamide at 55°C for 16 hr.

Thereafter, mice were placed in the small animal SPECT-CT scanner and carotid arteries were localized using contrast enhanced computed tomography of the neck region.

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