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The experiments on PTX3−/− mice were subsequent and separate from those on WT mice.
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While our initial correspondence with Kang and colleagues implied that two age ranges of mice were used, subsequent communication with Dr. Tae Won Kang has revealed that for the particular experiments outlined in this replication, all mice should be 4 6 weeks of age.
There were subsequent recriminations.
Two independent lines of TRE-Id1 mice were used for subsequent analysis.
Progeny were screened by genotyping, and PKD1flox/- OMPcre (OE null), and PKD1flox/+ OMPcre (OE heterozygote) mice were used for subsequent experiments.
For analysis of cultured neurons, 50 µl aliquots containing acutely dissociated cells (n = 6 mice) were used for subsequent qRT-PCR analysis.
Only male mice were used in subsequent experiments.
Following application mice were euthanized at subsequent time points and the application site and draining lymph nodes were dissected.
Therefore, atrial cells from 8-week-old mice were used in subsequent experiments (n = 3 per experiment), allowing for a marked abbreviation of the sorting procedure.
EdU-labeled mice were subjected to subsequent 5-bromo-2′-deoxyuridine administration to determine whether EdU-labeled cells could re-enter the cell cycle.
Male mice were used in all subsequent experiments.
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