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Tissue sections from saline and S. pneumoniae infected mice were next analyzed by histology.

Tissue sections from saline and infected mice were next analyzed to assess changes in lung histology and collagen deposition.

Tissue sections from S. pneumoniae-infected WT and PAI-1−/− mice were next stained for fibrin (ogen) by IHC (Fig. 8c).

Tissue sections from saline and S. pneumoniae-infected mice were next stained for fibrin ogen) by immunohistochemistry (red stain, Fig. 4b).

Splenocytes from WT mice were next tested for chemotaxis to murine CX3CL1 and CXCL12 as control.

To determine the functional significance of Vhl in α-cells, the Glu-Cre transgenic mice were next bred with mice in which exons 2 and 3 of the Vhl alleles are flanked by loxP sites (flox or f) [25].

Similar experiments soon followed with rats [26], and isolated mouse spermatogonia were next shown to maintain their regenerative potential after months in culture [27].

To further increase stress to the DBA/1 mice, female mice were put next to them, also in cages without filter top.

The constructed mutants were next injected intravenously in mice and mice survival was recorded over time.

The pathogenicity and pandemic potential of the virus were next examined in mice and ferrets, the established mammalian surrogates for humans in influenza research.

The strains were next injected intravenously in mice and tissue burden were next assessed from kidneys and spleen from sacrificed animals (Fig. 8).